Plasmodium vivax is the most widely dispersed malaria parasite worldwide. Transmitted by an Anopheles spp. female mosquito bite, this parasite is responsible for the majority of malaria cases in Brazil. One of the major difficulties in facing vivax malaria elimination is the fact that gametocytes can be found at the blood circulation in the very beginning of the infection. Therefore, it is necessary to have a clearer view of the gametocyte biology and the immune response towards to the antigens present at this stage. For such purpose, gametocytes proteins were analyzed in silico and Pvs047 was chosen as a predicted antigenic protein. The sexual antigen was recombinant expressed in eukaryotic cells, purified and its antigenicity was evaluated in a set of individuals infected with P. vivax, from Manaus, North of Brazil. Our results indicated that this protein is antigenic and 13.3% of the study population had IgG antibodies against Pvs047 while only 5.55% had IgM antibodies to this antigen. More results are needed to better understand the immune response to gametocytes antigens and therefore to build new strategies to eliminate P. vivax.